This study evaluated the effects of varying dietary lipid sources on growth performance, feed utilization, and carcass composition of Labeo rohita. Three experimental diets, including a control diet (CD), a low omega fat diet (LOFD), and a high omega fat diet (HOFD), were prepared and fed to fish for three months. Water quality parameters remained within the optimal range for fish culture throughout the experiment, with no significant differences among treatments. Growth performance indices, including final weight, weight gain, average daily gain (ADG), specific growth rate (SGR), and feed conversion ratio (FCR), were significantly influenced by dietary lipid source. The LOFD group achieved the highest growth performance and feed utilization efficiency. Proximate composition analysis revealed significantly higher crude protein and lipid content in the LOFD group, and no notable changes in ash content among the dietary groups. These findings indicate that moderate lipid in combination with vegetable oil and omega fat optimizes growth and carcass composition in L. rohita, providing valuable insights for formulating efficient and sustainable aquafeeds.